DNA extraction or DNA isolation is a process by which you can take out DNA from a sample through an amalgamation of varied physical and chemical techniques. This entire notion was innovated in the year 1869 and at present, is conducted as a routine procedure for forensic investigations and in the sectors of molecular biology.
DNA extraction consists of the following steps:
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DNA extraction consists of the following steps:
- Collecting the cells.
- Breaking the membranes so that the DNA and cytoplasm could be exposed.
- The solution is treated with salt (concentrated) so that the unnecessary debris like lipids, broken proteins, and RNA could clump together.
- Centrifugation is conducted so that cellular debris could be separated from the DNA.
- The extracted DNA is purified either by using ethanol or phenol-chloroform.
- The said method is used for genetically modifying plants. A large number of top-notch agricultural companies are noted to utilize the extraction procedure for creating DNA, which they then alter so that a crop of specific genetic strain could be generated. This new product can have diverse beneficial features like resistance to pests and chemicals. For example, a number of seeds are produced lately that are not affected by herbicides. As a result, farmers can spray herbicides all over the field to kill unwanted weeds but not harm the main crop.3
- Researchers have quite effectually manifested that DNA extraction stands to be extremely useful when it comes to animal genetic engineering. Well, the stated domain is extensive and consists of varied categories. It can be as simple as altering a single gene so as to make an animal with at least one new characteristic trait. For example, pigs that can glow in dark. The more complicated process included in genetic engineering is cloning through which absolutely identical creatures could be made.
- DNA extraction is one of the foremost techniques used to manufacture a series of pharmaceuticals. Medications that are produced through recombinant genetics contain human growth hormone (hGh) and bovine growth hormone (bVh). The most common example in this case is insulin, which controls the metabolism of fats, carbohydrates, and proteins by triggering the absorption of glucose from liver, blood, and skeletal muscular cells.
- Plenty of medical conditions could be diagnosed by extracting DNA from the patient’s body. Some such issues include sickle-cell anemia, cystic fibrosis, Huntington’s disease, fragile x syndrome, hemophilia, and Tay-Sachs disease. In addition, this method could also be utilized to check if a person is a carrier of certain ailment but does not have it himself.
- Finally, yet importantly, DNA extraction could be used to detect and verify identities. The most common process is fingerprinting that can place an individual at a crime scene as well as exonerate someone, who is convicted falsely of anoffense.
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